Improvement of Motor and Sensory–Motor Behaviors after the Human Amniotic Epithelial Cells Transplantation in 6-OHDA Lesioned Corpus Striatum of Wistar Albino Rats

Abstract

Deficits in apomorphine-induced rotation and rotarod in rat have been proposed as a nondrug-induced model of the akinesia associated with the Parkinson’s disease. In the present study, we examined the disabilities in the rotarod and apomorphine-induced rotations have been observed after the 6-hydroxydopamine-induced denervation in the striatum. These impaired activities have reversed after the Human Amniotic Epithelial (HAE) cells transplantation during long-term observation. The neuronal densities and diameter were reduced in the 6-OHDA-infused striatum and the densities and diameter of the neurons were slightly increased after the HAE cells transplanted striatum. The HAE cells did not have any surface antigen to create the graft-versus-host disease.  HAE cells from term human placenta express surface markers and genes characteristic of embryonic stem cells and have the ability to differentiate into all three germ layers, including tissues of endodermal origin i.e., liver. HAE cells possess the ability to differentiate into cells with characteristics of functional hepatocytes, in vitro and in vivo, thus representing a useful and noncontroversial source of cells for transplantation. In the present investigation, the HAE cells transplanted animals showed improvement on the motor, sensorimotor and muscular coordination.

Keywords: Human amniotic epithelial cells, Apomorphine-induced rotation, DiI labelling, Rotarod, 6-OHDA lesion