Effective Antibiotics and ATP Loaded Delivery System in the Management of Diabetic Wounds

Abstract

The aim of present research work was to formulate and evaluate antibiotics and ATP-loaded delivery system (liposome) in the management of diabetic wounds. Wound is disturbance in the normal skin anatomy and their functions; tissue injury resulting in the “loss of continuity of epithelium with or without the loss of underlying connective tissue.” Wound bad is an ultimate situation for wound infection, while wound healing is a complex and dynamic process of restoring cellular structures and tissue layers in which growth factors and cytokines play a momentous role. Impaired wound healing is a major clinical problem in patients with diabetes. Studies have shown delayed wound healing in diabetes due to cell proliferation deficiency, infection, decreased contraction, abnormalities in inflammatory cells response, impaired neovascularization, decreased synthesis of collagen and increased levels of proteases. Adenosine-5'-triphosphate (ATP) is a multifunctional nucleotide used in cells as a coenzyme. Antibiotic-loaded Nano carriers are a crucial element of standard medical procedures for both local treatment as well as prevention of wound infections. Cationic liposomes were characterized for an assortment of parameters together with shape and surface morphology, vesicle size and size distribution, drug-entrapment efficiency, zeta potential and in vitro drug release. Percentage entrapment efficiency was found to be 72.66 ± 2.3% for TC and 42.57 ± 2.1 for ATP. The typical vesicle size and PDI were found to be 194.6 nm and 0.281 respectively.