Phenetic Analysis of Solanum melongena Accessions to Explore the Genetic Diversity by SDS-PAGE

Abstract

The present experimental work was conducted for the phenetic analysis among 40 accessions of Solanum melongena from different geographical areas of Pakistan. The interspecific variation was found among the accessions of S. melongena. The analysis of seed storage was done by SDS-PAGE to check their relationship. Electrophoretic data was documented by gel documentation system (Bio-Rad, USA), and analyzed by using quantity 1-D analysis software and dendogram constructed with 100% tolerance in UPGMA (unweight pair-group arithmetic mean). The electrophorogram of accession Nos. (1–19) 018477, 018482 (Faisalabad) and 18484 (Sahiwal) was unique in protein banding position as compared to other accessions with light and dark bands. The band numbers and molecular weight of these were 7(49 KDa – 018477acc.), 18(21 KDa – 018482acc.) and 13(36 KDa) in 08184 accession from Sahiwal. The accessions 20–40 also have the unique banding pattern; band No. 2(70 KDa) from D. I. Khan (18504), Sahiwal (20344), and Batgram (20509). Band No. 7(49 KDa–18500, 18505, 14466(3) acc.) belonging to D. I. Khan and Bhawalpur was also having unique protein profile as well as 17(24KDa–20281, 20425, 44663) band was also unique in position. This work has proved that the accessions have low level of genetic diversity, which was negligible. Constructed dendogram has shown cluster 1 divided into 6(6a, 6b), 7(7a, 7b), 8(8a, 8b) and 9(9a, 9b) was the largest cluster including accessions 20425-4745(3), which belong to different geographical areas, but have no genetic variations. So, according to results, the all studied accessions had almost similar protein contents.