Phenotypic detection of ESBL production in Escherichia coli isolated from UTI patients of Kalaburagi city, Karnataka, India

Abstract

Aim: The principle aim of present conducted study was phenotypic detection of extended spectrum ß-lactamase (ESBL) production in Escherichia coli strains isolated from urinary tract infected patients of Kalaburagi city, Karnataka, India.

Materials and Methods: The positive E. coli isolates selected for the following study was isolated from urine sample of UTI patients. The screening of positive E. coli was done and antibiotic susceptibility test and MIC were determined as per CLSI guidelines. Phenotypic detection of ESBL production in E. coli isolates was determined by combination disk method and double disc synergy test. The positive ESBL production was determined based on the zone of inhibition by comparing with standard CLSI guidelines.

Results: Among 285 E. coli isolates, 268 (94.03%) isolates were multidrug resistant. Among 268 MDR E. coli isolates, 247(92.16 %) isolates were resistant to cephalosporins. Every 247 cephalosporin resistant E. coli isolates have shown high level of MIC range of ≤256 µg/ml. Among 247 E. coli isolates, 187 (75.70%) isolates were positive for ESBL production. Among 187 positive ESBL, 67 (35.8%) were cefetoxime-mediated ESBL producers, 78 (41.71%) E. coli isolates were ceftazidime-mediated ESBL producers and subsequently 52 (27.8%) were ceftriaxone-mediated ESBL producers.

Conclusion: The outcome of the present study, increased incidence of ESBL producing E. coli causes UTI, in hospitals and the E. coli isolates were MDR and responsible for ESBL production against majority of cephalosporin group of antibiotics, preferentially used for the treatment of gram negative bacterial infections.

Keywords: E. coli, ESBL, UTI, MDR, MIC

Cite this Article

Siddarth Surwonse, Rahul Narasanna, Kelmani Chandrakanth R et al. Phenotypic Detection of ESBL Production in Escherichia coli Isolated from UTI Patients of Kalaburagi City, Karnataka, India. Research & Reviews: Journal of Microbiology and Virology. 2017; 7(2): 1–6p.