Diagnosis of Dengue by NS1 Detection: Comparison of Rapid Immunochromatography versus ELISA Kits

Abstract

Dengue is an acute infectious disease of viral etiology. It is probably one of the most important arthropod borne viral disease in terms of human morbidity and mortality. The spectrum of disease ranges from self-limited dengue fever to more severe forms of dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). So, to reduce mortality and morbidity early and accurate diagnosis of dengue is essential. Study assessed comparison of currently available rapid test for NS1, IgG, and IgM combo kit and conventional dengue Ag ELISA test (CTK Biotech) ELISA kit. The performance of rapid kit RICT (Dengue Day 1 Test; J. Mitra) is quite satisfactory as sensitivity of test as compared to ELISA test was 94.44%. Regarding specificity, with reference to dengue Ag ELISA test (CTK Biotech), the specificity of RICT (Dengue Day 1 test; J. Mitra) was 71.43%. The use of combination kit helps to detect additional cases of dengue, which are negative for NS1 antigen but positive for IgM and/or IgG antibodies, thus facilitating early diagnosis in remote areas and small laboratories.

Keywords: Dengue, NS1, ELISA, immunochromatography

Cite this Article

Gaurav Saraf, Vasudev Patidar, Asha Sharma, Pushpa Mehta. Diagnosis of Dengue by NS1 Detection: Comparison of Rapid Immunochromatography versus ELISA Kits. Research & Reviews: Journal of Microbiology and Virology. 2016; 6(2): 18–22p.