Study of Aerobic and Anaerobic Bacteriological Profile in Patients Presenting with Ear Discharge and Antimicrobial Susceptibility Pattern of Aerobic Isolates

Abstract

Ear discharge is a major health problem in both, developed and developing countries. This study was undertaken to determine the pattern of the different etiological aerobic and anaerobic bacteria in patients presenting with ear discharge and clinically diagnosed as CSOM and ASOM and also to know the antibiogram of aerobic bacteria in Kota Rajasthan. A prospective study was carried out in department of microbiology, MBS Hospital, Kota, among 100 patients attending the ENT OPD over a period of 12 months. Informed consent was obtained from the patients for this study and microbiological analysis was done. Ear swabs were taken from 100 patients. Total 108 swabs were collected as some patients presented with bilateral ear discharge, the total number of bacterial isolates were 109 as some swabs showed polymicrobial growth and six cases were sterile. Gram-negative bacteria were the dominant isolates (69.20%) while gram-positive bacteria were 32 (30.76%). The most common isolate overall among both the CSOM and ASOM was Pseudomonas aeruginosa (42.20%), followed by Staphylococcus aureus (27.52%), Proteus spp. (10.09%), Klebsiella spp. (6.42%), Escherichia coli (4.58%), Acinetobacter spp. (2.75%), Enterococcus spp. (1.83%), CONS (1.83%), Anaerobic gram-negative bacilli (1.83%) and anaerobic Gram-positive cocci (0.91%). Pseudomonas aeruginosa showed high-level resistance to antimicrobials. Least resistance was found for Amikacin, Ceftazidime, Imipenem, and Piperacillin/Tazobactam among gram-negative organisms and hence could be used for empirical therapy. Vancomycin (100%), Linezolid (100%) and Clindamycin (70%) were found to be most sensitive drugs for gram-positive organisms.

Keywords: CSOM, ASOM, aerobic, anaerobic, antimicrobial susceptibility

Cite this Article

Rashmi Sharma, Chand Anita E, Naruka HS, Dinesh Verma. Study of Aerobic and Anaerobic Bacteriological Profile in Patients Presenting with Ear Discharge and Antimicrobial Susceptibility Pattern of Aerobic Isolates. Research & Reviews: Journal of Microbiology and Virology. 2016; 6(2): 23–30p.