Frequency and Effect of CCR5 Alleles on HIV-1 Disease Progression among Untreated Seropositive Individuals Visiting Gondar University Teaching Hospital

Abstract

Abstract:
The natural history of HIV-1 infection varies considerably from one individual to another, with some individuals progressing to AIDS rapidly after primary infection, while others remain clinically asymptomatic with no evidence of immune dysfunction. Recent studies have shown that reasons for progression and non-progression are multi-factorial and may involve genetic, virological and immunological factors that influence HIV disease progression in various ways. Chemokine receptors act as important co-receptors mediating the entry of the human immunodeficiency virus type 1 (HIV-1) into susceptible cells. Homozygous genotype of CCR5 gene protects individuals from HIV-1 infection and the heterozygous form of the gene delays disease progression. The aim of the present study was to identify the frequency of CCR5-Δ32 (Chemochine receptor delta 32 allele) in HIV-1 untreated individuals and control groups. In this case, control study one hundred HIV-1 infected and untreated individuals and equal number of age- and sex-matched control groups were involved. Blood samples of both cases and controls were collected using EDTA coated non-vacutenous tubes. DNA was isolated using phenol-chloroform method and the PCR (polymerase chain reaction) was conducted to evaluate the frequency of the genotypes of CCR5. In this study, there was no mutant allele of CCR5 gene in both cases and controls. SPSS 17 was applied to evaluate the valuable data such as sex, age and occupation of study subjects. EPI info version 3.2 (Centre for Disease Control and Prevention) was used to see the association of the gene with HIV-1 susceptibility and disease progression. No statistically significant association was observed between CCR5 gene and HIV susceptibility in this case control study.

Keywords: HIV, AIDS, CCR5, disease progression

Cite this Article:

 Nega Berhane, Eyayaw Assefa. Frequency and effect of CCR5 alleles on HIV-1 disease progression among untreated seropositive individuals visiting Gondar University teaching hospital. Research and Reviews: Journal of Microbiology and Virology. 2015; 5(1): 8–13p.