Chemical Profiling and Antibacterial Activity of Punica Granatum L against Pathogens Causing Bovine Mastitis

Abstract

The antibacterial activity of aqueous and methanol extracts of Punica granatum L was investigated against bovine mastitis causing bacterial pathogens- Streptococcus agalactiae, Staphylococcus aureus, Streptococcus uberis, Escherichia coli, Streptococcus dysagalactiae and coagulase negative Staphylococcus aureus by agar disc diffusion method [3] isolated locally from clinical cases. The results obtained in the present study scientifically proved and suggest the use of Punica granatum for treating mastitis causing organism. The phytochemical screening of the plant revealed the presence of Alkaloids, Tannins, Coumarins, Flavonoids, Steroids, Phenolic compounds, Saponins, Terpenoids, Catechin, Anthraquinone and Quinone. The proximate analysis was carried out. Antimicrobial activity of methanolic and aqueous extracts of flavonoids was done and its presence in methanolic and aqueous extracts of P. granatum was confirmed by TLC (Thin Layer Chromatography).

Keywords: Punica granatum, bovine mastitis pathogen, disc-diffusion method, Solvents, Antibacterial activity, Phyto-chemical screening, Proximate analysis