Helicobacter pylori Sialic Acid-Specific Surface Lectin Associated with Pathogenecity Island cag PAI

Abstract

Helicobacter pylori bacterium causing gastritis, duodenitis, non-ulcer dyspepsia, adenocarcinoma, MALT lymphoma and now-a-days causing hepatocellular carcinoma and cholengiocarcinoma has been studied for its surface antigens. There are many virulence factors which have been studied since many years. Cag-PAI is the site for virulence and causes mixed infections. Sialic acid is a type of glycan which is a surface lectin associated with adherence to cell lines and other virulence factors studied in detail in H. pylori. Sialic acid dependency is been studied by performing heamagglutination studies. Sialidase activity was carried out by haemagglutination assay on H. pylori. Some strains were found Sia-dependent and many were found Sia-independent. CagA, VacA, VirD4 genes were identified by PCR in some strains and picB gene was not detected in the 19/20 strains analysed. Thus, it was found that Sia-dependent and Sia-independent strains of H. pylori were correlating with PCR strains analysed for virulent factors. H. pylori strains where in total 9/54 strains were Sia-dependent belonged to class I category and others to class II category. Haemagglutination and PCR analysis revealed pathogenecity in some strains. Therefore, if broad spectrum studies are carried out on a large scale, it will be very useful in the diagnosis and prognosis of the diseases caused by H. pylori.

Keywords: H. pylori, haemagglutination, sialic acid, PCR, cag PAI

Cite this Article

Rupa Mulchandani, Hans-Olof Nilsson,  Torkel Wadström. Helicobacter pylori Sialic Acid-Specific Surface Lectin Associated with pathogenecity Island Cag PAI. Research & Reviews: Journal of Microbiology and Virology. 2015; 5(3): 10–17p.