Isolation and Characterization of Protease Producing Organism Bacillus Species from Slaughter House Waste

Abstract

The purpose of this investigation was to isolate protease producing bacteria from slaughter house waste collected in local area of Kaliakkavilai. The Bacillus sp. was identified by several biochemical and physiological tests. The effect of different production parameters such as pH, temperature, metal ions concentration and substrate concentration for protease production by the isolated bacterial strain were studied. The enzyme production was assayed in submerged fermentation with optimized parameters to produce maximum protease activity and specific activity was observed as 1.067 and 0.011 µmol/ml/min. The partial purification of protease was done by column chromatography.

Keywords: Slaughter house, protease enzyme, column chromatography, Bacillus sp.