Preparation of Lipase Loaded Chitosan Nanoparticles and its Estimation Using Different Additives

Abstract

Chitosan nanoparticles were prepared by ionic gelation technique and simultaneously entrapment of lipase was carried out in it. These were then subjected to additives such as surfactants, pluronics and ionic liquids. Activity of lipase was assayed in free as well as immobilized conditions. It was then compared to immobilized enzyme bound to these polymers. The results have been graphically displayed. Among surfactants, tween 80 and triton X-100 were used with the latter showing better activity. Pluronic P127 showed maximum activity of the enzyme and ionic liquid hexadecyl pyridinium bromide displayed best results. Stability of chitosan nanoparticles was observed on combining these with the additives.

Keywords: Chitosan nanoparticles, lipase activity, surfactants, ionic liquids, pluronics

Cite this Article

Devnani H, Dani U, Bahadur A. Preparation of Lipase Loaded Chitosan Nanoparticles and its Estimation Using Different Additives. Research & Reviews: A Journal of Life Science. 2016; 6(2): 19–27p.