Insecticidal Activity of Rotenone in Brebra (Millettia Ferruginea) Oil Refinery Residue against Different Stored Crop Insect Pests and its Prospect to Apply as Pest Control Substance

Abstract

Use of botanical insecticides against stored crop insect pests is environmental safer and eco-friend alternative method for crop protection. The objective was to investigate insecticidal potential of oil refinery residue with rotenone against selected insect pest of stored crops. The amount of rotenone from samples was determined by HPLC. The toxicity of the rotenone from residual extract and non-defatted sample were tested against. Acanthoscelides obtectus, Sitophilus zeamais and Tribolium castaneum using standard methods. 1, 1.5 and 2 ml residue contains 1037.089, 1555.98 and 2075.57 μg rotenone, respectively. Significant differences (P ≤ 0.01) were observed among different doses of treatment for % mortality of Tribolium castanium and Sitophilus zeamais after 24 h of treatment but, there was no significant difference (P ≤ 0.01) at doses of 2 ml of residues against Tribolium castanium and 1.5 ml against Sitophilus zeamais after treatment of 24 h. Tribolium castanium seems to be more resistant than Sitophilus zeamais and Acanthoscelides obtectus for rotenone from oil refinery residue. Any way 2 ml of oil residue has potential to kill 100% of tested pests after 24 h of treatment. Therefore, the toxicity of the different extracts to tested insects was dose dependent, however, the mortality as compared to control was significant (P<0.001). So, residue from refined oil has huge potential of insecticidal activity and can serve as insect pesticide at high concentration level. It is economically important for insect crop pest control in one hand and save the money spent for inorganic insect pest chemicals and thereby reduces environmental impact of non-degradable chemicals.
Keywords: Insecticides, insect pests, Millettia ferruginea, refinery residue, rotenone, stored crop